About this 3D model

Application: - Reduce antibody costs by up to 10-fold - Perform IF staining with 5-20 µl of antibody/blocking solution per well - Perform FISH with 20 µl volumes - Enables whole plate staining on glass-bottomed plates Parameters: Material: PLA Operating temperature: < 150 °C Autoclavable: No Clean using 70% and 100% ethanol. Distance between well centres: 26 mm Design basis: Greiner Bio-one Cellstar 12-well plate Protocol: Minor modifications to staining protocols may be necessary. During testing, we established that the columns retain heat after FISH incubation for 10 min at 80 °C. The plate should be immediately cooled at 4 °C to prevent slow reannealing of DNA and to improve signal to noise ratio. More stringent post-FISH wash with lower SSC buffer concentration should be performed to reduce cytoplasmic background staining. Modifications: Users may attach glass coverslips or aluminium foil to the base of each column to create a flatter surface and promote ease of cleaning.